Journal: Cell reports

Article Title: Btla signaling in conventional and regulatory lymphocytes coordinately tempers humoral immunity in the intestinal mucosa

doi: 10.1016/j.celrep.2022.110553

Figure Lengend Snippet: (A and B) Plots and graphs of Foxp3 − PD1 + Cxcr5 + Tfh, Foxp3 + PD1 − Cxcr5 − Treg, and Foxp3 + PD1 + Cxcr5 + Tfr cells (A) and IgD low CD38 hi memory B cells, IgD low CD38 low GL7 + Fas + GC B cells, GC B cell cellularity, and PP cellularity (B) in PPs of 8-week-old Btla flox , Btla ΔCd4 , and Btla ΔCd19 animals. (C) PP sections from 8-week-old Btla flox and Btla ΔCd4 animals stained with anti-CD4, anti-B220, anti-IgA, and DAPI. Scale bar equals 100 μm. (D) Plots of Cxcr4 hi CD86 lo DZ and Cxcr4 lo CD86 hi LZ cells and graph of DZ/LZ cell ratio in PPs of 8-week-old Btla flox , Btla ΔCd4 , and Btla ΔCd19 animals. Data analyzed using multi-parameter linear modeling ANOVA. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. Representative of three separate experiments with n = 4 replicates for each condition. See also – .

Article Snippet: For intracellular staining, after surface staining, cells were fixed using the Foxp3 transcription factor staining kit Fixation/Permeabilization buffer (ThermoFisher) for 20 minutes on ice.

Techniques: Staining

Journal: Cell reports

Article Title: Btla signaling in conventional and regulatory lymphocytes coordinately tempers humoral immunity in the intestinal mucosa

doi: 10.1016/j.celrep.2022.110553

Figure Lengend Snippet: (A and B) Plots and graphs of Foxp3 − PD1 + Cxcr5 + Tfh, Foxp3 + PD1 − Cxcr5 − Treg, and Foxp3 + PD1 + Cxcr5 + Tfr cells (A) and IgD low CD38 low GL7 + Fas + GC B cells, GC B cellularity, and PP cellularity in PPs of 8-week-old Tnfrsf14 flox , Tnfrsf14 ΔCd4 , and Tnfrsf14 ΔCd19 animals (B). (C) PP sections from 8-week-old Tnfrsf14 flox and Tnfrsf14 ΔCd19 animals stained with anti-CD4, anti-B220, anti-IgA, and DAPI. Scale bar equals 100 μm. (D) Plots of Cxcr4 hi CD86 lo DZ and Cxcr4 lo CD86 hi LZ cells and graph of DZ/LZ cell ratio in PPs of 8-week-old Tnfrsf14 flox , Tnfrsf14 ΔCd4 , and Tnfrsf14 ΔCd19 animals. Data analyzed using multi-parameter linear modeling ANOVA. *p < 0.05; **p < 0.01; ***p < 0.001. Representative of three separate experiments with n = 4 replicates for each condition. See also and .

Article Snippet: For intracellular staining, after surface staining, cells were fixed using the Foxp3 transcription factor staining kit Fixation/Permeabilization buffer (ThermoFisher) for 20 minutes on ice.

Techniques: Staining

Journal: Cell reports

Article Title: Btla signaling in conventional and regulatory lymphocytes coordinately tempers humoral immunity in the intestinal mucosa

doi: 10.1016/j.celrep.2022.110553

Figure Lengend Snippet: (A–C) Graphs of T cell subsets in PPs of control or anti-Btla-treated wild-type (A), Tnfrsfl14 −/− (B) animals, or of untreated Btla flox and Btla ΔFoxp3 animals (C). (D) Plots and graph of FoxP3 + YFP +/− Treg cells in PPs of untreated wild-type Foxp3 +/cre and Btla flox/flox Foxp3 +/cre animals. (E and F) Plots and graphs of Foxp3 − PD1 + Cxcr5 + Tfh, Foxp3 + PD1 − Cxcr5 − Treg, and Foxp3 + PD1 + Cxcr5 + Tfr cells (E) and IgD low CD38 low GL7 + Fas + GC B cells (F) in PPs of untreated wild-type, Btla −/− , and tamoxifen-treated Btla flox and Btla ΔFoxp3ERT2 animals. Tam, yamoxifen. Data analyzed using multi-parameter linear modeling ANOVA. *p <0.05; **p<0.01; ***p < 0.001; ****p < 0.0001. Representative of six(wild-type) experiments, with at least n = 5 replicates for each condition; two ( Tnfrsf14 −/− ) experiments, with at least n = 7 replicates for each condition in antibody treatment experiments; and four (constitutive) experiments, with at least n = 3 replicates for each condition and two (induced) Treg cells Btla deletion experiments, with at least n = 8 replicates for each condition. See also and .

Article Snippet: For intracellular staining, after surface staining, cells were fixed using the Foxp3 transcription factor staining kit Fixation/Permeabilization buffer (ThermoFisher) for 20 minutes on ice.

Techniques: Control

Journal: Cell reports

Article Title: Btla signaling in conventional and regulatory lymphocytes coordinately tempers humoral immunity in the intestinal mucosa

doi: 10.1016/j.celrep.2022.110553

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: For intracellular staining, after surface staining, cells were fixed using the Foxp3 transcription factor staining kit Fixation/Permeabilization buffer (ThermoFisher) for 20 minutes on ice.

Techniques: Control, Plasmid Preparation, Recombinant, Cell Isolation, cDNA Synthesis, SYBR Green Assay, Staining, Enzyme-linked Immunosorbent Assay, Cloning, Library Quantification, Software